美国短毛黑水貂快速生长过程中转录组差异表达分析

doi:10.11843/j.issn.0366-6964.2019.09.006

Abstract

Abstract: This study aimed to analyze the differential expression genes based on transcriptome during the rapid growth process of American short-hair black mink. In this study, the breast muscle of 45 and 90 days old healthy American short-hair black mink (3 independent repetitions) were used as experimental material, the transcriptome library was established using Illumina HiSeq^TM2500 high-throughput sequencing platform. GO and KEGG analysis were performed on the differential expression genes between the two stages, and the candidate genes and metabolic pathways regulating the rapid growth of mink were screened. qRT-PCR was used to validate the result of transcriptome sequencing. The results showed that 279 significant differentially expressed genes were screened out with P<0.05 and|log₂FC|>1 as the criterion. GO enrichment analysis of these genes showed that 66 GO terms were significantly enriched and 44 differentially expressed genes were related to muscle growth, among which 20 were up-regulated and 24 were down-regulated. Twelve pathways related to muscle growth were screened out, such as p53, PPAR and FOXO signaling transduction pathways. These genes may play a regulatory role in the rapid growth process of mink, and can be used as candidate genes for the further study on the growth and development of mink. The results could provide a theoretical basis for exploring the regulation mechanism of growth and development in mink, and breeding large-size mink breeds.

CLC Number:

S865.22

RONG Min, ZHANG Ranran, TU Jianfeng, WANG Tianjiao, SUN Limin, YANG Ying, XU Jiaping, XING Xiumei*. American Short-hair Black Mink Transcriptome Differential Expression Genes Analysis During the Rapid Growth Process[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1787-1801.

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American Short-hair Black Mink Transcriptome Differential Expression Genes Analysis During the Rapid Growth Process

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